Lysosomal Membrane and Protein Stabilization by Dalbergia Sissoo (family: Fabaceae): in Vitro Anti-inflammatory Activity

Objective: Plants of the genus Dalbergia are reported to be useful in the treatment of arthritis, gonorrhoea and rheumatic pains. Present study was aimed to investigate the in vitro anti-inflammatory activity of ethanol extract from Dalbergia sissoo leaves (EDS) and to support its traditional use. Methods: EDS was investigated for it's in vitro anti-inflammatory activity in human red blood cell membrane stabilization (HRBC) method and protein denaturation method. Diclofenac sodium was used as the standard drug. Results: The EDS and diclofenac sodium showed a concentration dependent stabilization toward HRBC membrane with 314.3±0.01 and 34.91±0.01 μg/ml; 50% protection, respectively. EDS and diclofenac sodium also showed dose dependent protein denaturation with IC50 Conclusions: EDS possessed noticeable in vitro anti-inflammatory effect against the HRBC membrane stabilization method and denaturation of albumin. Further authoritative studies are necessary to make certain the mechanisms and constituents behind its anti-inflammatory actions. values 719.9±0.04 and 428.4±0.02 μg/ml, respectively.